The MyoD1 Polyclonal Antibody (CAB0671) is a crucial tool for researchers studying the MyoD1 protein, a key regulator of myogenic differentiation and muscle development. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MyoD1 protein, this antibody allows for accurate and reliable detection and analysis in a variety of cell types, making it an essential component for investigations in muscle biology and tissue regeneration.
MyoD1, also known as Myogenic Differentiation 1, is essential for the formation of skeletal muscle and plays a pivotal role in the differentiation of myoblasts into mature muscle cells. Dysregulation of MyoD1 expression has been linked to various muscle disorders and diseases, making it a promising target for research into muscle regeneration therapies and treatments for muscle-wasting conditions. By elucidating the functions and mechanisms of MyoD1, researchers can pave the way for novel therapeutic approaches in muscle-related pathologies.
Product Name:
MYOD1 Rabbit Polyclonal Antibody
SKU:
CAB0671
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_002469.2).
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Purification Method:
Affinity purification
Gene ID:
4654
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb(CAB0671) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
