The Myo15A Polyclonal Antibody (PACO64319) is an essential tool for research involving Myo15A, a protein associated with the development and function of the inner ear. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards Myo15A in human samples, making it a reliable choice for various experimental applications such as Western blotting. By targeting the Myo15A protein, researchers can accurately detect and analyze its expression in different cell types, making it a valuable tool for studies in otology and hearing research.Myo15A, also known as myosin XVA, plays a crucial role in the formation and maintenance of hair bundles in the cochlea, contributing to auditory sensory function. Mutations in the Myo15A gene have been linked to various forms of genetic hearing loss, making it a target of interest in research on deafness and auditory disorders.
By investigating the function and regulation of Myo15A, researchers can gain insights into the mechanisms underlying hearing loss and potentially identify novel therapeutic targets for hearing-related conditions.Overall, the Myo15A Polyclonal Antibody (PACO64319) serves as a valuable tool for scientists conducting research in the field of auditory biology, providing a means to study the role of Myo15A in inner ear development and function. Its specificity and reliability make it ideal for investigating the molecular mechanisms underlying hearing loss and exploring potential therapeutic interventions for auditory disorders.
Antibody Name:
MYO15A Antibody (PACO64319)
Antibody SKU:
PACO64319
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
IF:1:100-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Unconventional myosin-XV protein (237-451AA)
Immunofluorescence staining of MCF-7 cells with PACO64319 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Required for the arrangement of stereocilia in mature hair bundles (By similarity).
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Required for the arrangement of stereocilia in mature hair bundles ().
UniProt Protein Details:
NCBI Summary:
This gene encodes an unconventional myosin. This protein differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in this gene have been associated with profound, congenital, neurosensory, nonsyndromal deafness. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and this gene have been identified, but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described, but their full length sequences have not been determined. [provided by RefSeq, Jul 2008]
