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Anti-MYL2 Antibody (CAB5473)

SKU:
CAB5473
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 9 Anti-MYL2 Antibody CAB5473
  • Cell Biology Antibodies 9 Anti-MYL2 Antibody CAB5473
  • Cell Biology Antibodies 9 Anti-MYL2 Antibody CAB5473
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Description

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Anti-MYL2 Antibody (CAB5473)

The MYL2 Polyclonal Antibody (CAB5473) is a valuable tool for researchers studying MYL2, a key protein involved in muscle contraction. This antibody, produced in rabbits, exhibits high reactivity with samples from various species, including human samples. Validated for use in Western blot applications, this antibody specifically targets the MYL2 protein, enabling accurate detection and analysis in different cell types.MYL2, also known as myosin light chain 2, plays a crucial role in regulating muscle contraction and function. Dysregulation of MYL2 has been linked to various cardiac and skeletal muscle disorders, making it a significant target for research in cardiovascular and muscle-related diseases.

Understanding the intricate workings of MYL2 is essential for developing targeted therapies and interventions for these conditions.Overall, the MYL2 Polyclonal Antibody is a reliable tool for researchers investigating the role of MYL2 in muscle physiology and pathology. Its specificity and sensitivity make it an ideal choice for studies in the fields of cardiology, muscle biology, and potential drug development.

Antibody Name:Anti-MYL2 Antibody
Antibody SKU:CAB5473
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPK KAKK RAGG ANSN VFSM FEQT QIQE FKEA FTIM DQNR DGFI DKND LRDT FAAL GRVN VKNE EIDE MIKE APGP INFT VFLT MFGE KLKG ADPE ETIL NAFK VFDP EGKG VLKA DYVR EMLT TQAE RFSK EEVD QMFA AFPP DVTG NLDY KNLV HIIT HGEE KD
Gene ID:4633
Uniprot:P10916
Cellular Location:A band, Cytoplasm, myofibril, sarcomere
Calculated MW:18kDa
Observed MW:16kDa
Synonyms:MYL2, CMH10, MLC-2s/v, MLC2
Background:Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
UniProt Protein Function:MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
UniProt Protein Details:

Protein type:Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton

Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development

Disease: Cardiomyopathy, Familial Hypertrophic, 10

NCBI Summary:Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Code:P10916
NCBI GenInfo Identifier:6166556
NCBI Gene ID:4633
NCBI Accession:P10916.3
UniProt Secondary Accession:P10916,Q16123,
UniProt Related Accession:P10916
Molecular Weight:166
NCBI Full Name:Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Synonym Full Names:myosin, light chain 2, regulatory, cardiac, slow
NCBI Official Symbol:MYL2  
NCBI Official Synonym Symbols:MLC2; CMH10  
NCBI Protein Information:myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
UniProt Protein Name:Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family:Myosin regulatory
UniProt Gene Name:MYL2  
UniProt Entry Name:MLRV_HUMAN
Anti-MYL2 Antibody (CAB5473)Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB5473) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MYL2 Antibody (CAB5473)Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-MYL2 Antibody (CAB5473)Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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