Anti-MYL2 Antibody (CAB5473)
- SKU:
- CAB5473
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-MYL2 Antibody (CAB5473)
The MYL2 Polyclonal Antibody (CAB5473) is a valuable tool for researchers studying MYL2, a key protein involved in muscle contraction. This antibody, produced in rabbits, exhibits high reactivity with samples from various species, including human samples. Validated for use in Western blot applications, this antibody specifically targets the MYL2 protein, enabling accurate detection and analysis in different cell types.MYL2, also known as myosin light chain 2, plays a crucial role in regulating muscle contraction and function. Dysregulation of MYL2 has been linked to various cardiac and skeletal muscle disorders, making it a significant target for research in cardiovascular and muscle-related diseases.
Understanding the intricate workings of MYL2 is essential for developing targeted therapies and interventions for these conditions.Overall, the MYL2 Polyclonal Antibody is a reliable tool for researchers investigating the role of MYL2 in muscle physiology and pathology. Its specificity and sensitivity make it an ideal choice for studies in the fields of cardiology, muscle biology, and potential drug development.
| Antibody Name: | Anti-MYL2 Antibody |
| Antibody SKU: | CAB5473 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPK KAKK RAGG ANSN VFSM FEQT QIQE FKEA FTIM DQNR DGFI DKND LRDT FAAL GRVN VKNE EIDE MIKE APGP INFT VFLT MFGE KLKG ADPE ETIL NAFK VFDP EGKG VLKA DYVR EMLT TQAE RFSK EEVD QMFA AFPP DVTG NLDY KNLV HIIT HGEE KD |
| Gene ID: | 4633 |
| Uniprot: | P10916 |
| Cellular Location: | A band, Cytoplasm, myofibril, sarcomere |
| Calculated MW: | 18kDa |
| Observed MW: | 16kDa |
| Synonyms: | MYL2, CMH10, MLC-2s/v, MLC2 |
| Background: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Function: | MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Details: | Protein type:Contractile Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development Disease: Cardiomyopathy, Familial Hypertrophic, 10 |
| NCBI Summary: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P10916 |
| NCBI GenInfo Identifier: | 6166556 |
| NCBI Gene ID: | 4633 |
| NCBI Accession: | P10916.3 |
| UniProt Secondary Accession: | P10916,Q16123, |
| UniProt Related Accession: | P10916 |
| Molecular Weight: | 166 |
| NCBI Full Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| NCBI Synonym Full Names: | myosin, light chain 2, regulatory, cardiac, slow |
| NCBI Official Symbol: | MYL2Â Â |
| NCBI Official Synonym Symbols: | MLC2; CMH10Â Â |
| NCBI Protein Information: | myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow |
| UniProt Protein Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| Protein Family: | Myosin regulatory |
| UniProt Gene Name: | MYL2Â Â |
| UniProt Entry Name: | MLRV_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB5473) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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