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Anti-MYL12A Antibody (CAB9176)

SKU:
CAB9176
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$167 - $503
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Description

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Anti-MYL12A Antibody (CAB9176)

The MYL12A Polyclonal Antibody (CAB9176) is a valuable tool for researchers studying MYL12A, a key regulator of cell motility and contractility. This antibody, produced in rabbits, specifically targets the MYL12A protein and is highly reactive with human samples. Validated for use in Western blot applications, the MYL12A Polyclonal Antibody allows for the detection and analysis of MYL12A in various cell types.MYL12A, also known as myosin light chain 12A, plays a crucial role in the regulation of smooth muscle and non-muscle cell contractility. Dysregulation of MYL12A has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.

Therefore, studying the expression and function of MYL12A is essential for gaining insights into the underlying mechanisms of these diseases.With its high specificity and reactivity, the MYL12A Polyclonal Antibody is an essential tool for researchers conducting studies in cell biology, cancer research, and cardiovascular health. By enabling the detection and analysis of MYL12A, this antibody facilitates the exploration of potential therapeutic targets and biomarkers associated with MYL12A-related diseases.

Antibody Name:Anti-MYL12A Antibody
Antibody SKU:CAB9176
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_006462.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2, A375, HeLa, K-562, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_006462.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KLNG TDPE DVIR NAFA CFDE EATG TIQE DYLR ELLT TMGD RFTD EEVD ELYR EAPI DKKG NFNY IEFT RILK HGAK DKDD
Gene ID:10627
Uniprot:P19105
Cellular Location:
Calculated MW:19kDa
Observed MW:18kDa
Synonyms:MYL12A, HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B
Background:This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
UniProt Protein Function:MRLC3: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains.
UniProt Protein Details:

Protein type:Contractile

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: myosin II complex; stress fiber; cytosol; Z disc

Molecular Function:protein binding; glutamate receptor binding; calcium ion binding

Biological Process: axon guidance; regulation of cell shape; ephrin receptor signaling pathway

NCBI Summary:This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]
UniProt Code:P19105
NCBI GenInfo Identifier:127169
NCBI Gene ID:10627
NCBI Accession:P19105.2
UniProt Secondary Accession:P19105,Q53X45,
UniProt Related Accession:P19105
Molecular Weight:171
NCBI Full Name:Myosin regulatory light chain 12A
NCBI Synonym Full Names:myosin, light chain 12A, regulatory, non-sarcomeric
NCBI Official Symbol:MYL12A  
NCBI Official Synonym Symbols:MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24  
NCBI Protein Information:myosin regulatory light chain 12A; myosin RLC; myosin regulatory light chain 3; epididymis secretory protein Li 24; myosin regulatory light chain MRLC3; myosin regulatory light chain 2, nonsarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric
UniProt Protein Name:Myosin regulatory light chain 12A
UniProt Synonym Protein Names:Epididymis secretory protein Li 24; HEL-S-24; MLC-2B; Myosin RLC; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain MRLC3
Protein Family:C-type mannose receptor
UniProt Gene Name:MYL12A  
UniProt Entry Name:ML12A_HUMAN
Anti-MYL12A Polyclonal Antibody (CAB9176)Western blot analysis of extracts of various cell lines, using MYL12A antibody (CAB9176) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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