Anti-MYL12A Antibody (CAB9176)
- SKU:
- CAB9176
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-MYL12A Antibody (CAB9176)
The MYL12A Polyclonal Antibody (CAB9176) is a valuable tool for researchers studying MYL12A, a key regulator of cell motility and contractility. This antibody, produced in rabbits, specifically targets the MYL12A protein and is highly reactive with human samples. Validated for use in Western blot applications, the MYL12A Polyclonal Antibody allows for the detection and analysis of MYL12A in various cell types.MYL12A, also known as myosin light chain 12A, plays a crucial role in the regulation of smooth muscle and non-muscle cell contractility. Dysregulation of MYL12A has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.
Therefore, studying the expression and function of MYL12A is essential for gaining insights into the underlying mechanisms of these diseases.With its high specificity and reactivity, the MYL12A Polyclonal Antibody is an essential tool for researchers conducting studies in cell biology, cancer research, and cardiovascular health. By enabling the detection and analysis of MYL12A, this antibody facilitates the exploration of potential therapeutic targets and biomarkers associated with MYL12A-related diseases.
| Antibody Name: | Anti-MYL12A Antibody |
| Antibody SKU: | CAB9176 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_006462.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | LO2, A375, HeLa, K-562, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_006462.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KLNG TDPE DVIR NAFA CFDE EATG TIQE DYLR ELLT TMGD RFTD EEVD ELYR EAPI DKKG NFNY IEFT RILK HGAK DKDD |
| Gene ID: | 10627 |
| Uniprot: | P19105 |
| Cellular Location: | |
| Calculated MW: | 19kDa |
| Observed MW: | 18kDa |
| Synonyms: | MYL12A, HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B |
| Background: | This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. |
| UniProt Protein Function: | MRLC3: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. |
| UniProt Protein Details: | Protein type:Contractile Chromosomal Location of Human Ortholog: 18p11.31 Cellular Component: myosin II complex; stress fiber; cytosol; Z disc Molecular Function:protein binding; glutamate receptor binding; calcium ion binding Biological Process: axon guidance; regulation of cell shape; ephrin receptor signaling pathway |
| NCBI Summary: | This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014] |
| UniProt Code: | P19105 |
| NCBI GenInfo Identifier: | 127169 |
| NCBI Gene ID: | 10627 |
| NCBI Accession: | P19105.2 |
| UniProt Secondary Accession: | P19105,Q53X45, |
| UniProt Related Accession: | P19105 |
| Molecular Weight: | 171 |
| NCBI Full Name: | Myosin regulatory light chain 12A |
| NCBI Synonym Full Names: | myosin, light chain 12A, regulatory, non-sarcomeric |
| NCBI Official Symbol: | MYL12AÂ Â |
| NCBI Official Synonym Symbols: | MLCB; MRCL3; MRLC3; MYL2B; MLC-2B; HEL-S-24Â Â |
| NCBI Protein Information: | myosin regulatory light chain 12A; myosin RLC; myosin regulatory light chain 3; epididymis secretory protein Li 24; myosin regulatory light chain MRLC3; myosin regulatory light chain 2, nonsarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric |
| UniProt Protein Name: | Myosin regulatory light chain 12A |
| UniProt Synonym Protein Names: | Epididymis secretory protein Li 24; HEL-S-24; MLC-2B; Myosin RLC; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain MRLC3 |
| Protein Family: | C-type mannose receptor |
| UniProt Gene Name: | MYL12AÂ Â |
| UniProt Entry Name: | ML12A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL12A antibody (CAB9176) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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