The MYH7/MHC Monoclonal Antibody is a powerful tool for researchers studying the MYH7 gene, which encodes for the beta myosin heavy chain protein involved in muscle contraction. This antibody, produced using hybridoma technology, specifically targets the MYH7 protein and is ideal for use in various applications such as Western blot, immunofluorescence, and immunohistochemistry.The MYH7 protein is predominantly expressed in cardiac and skeletal muscle tissues, playing a crucial role in muscle contraction and overall muscle function. Mutations in the MYH7 gene have been linked to various muscle disorders, including cardiomyopathy and skeletal muscle myopathies.
By using the MYH7/MHC Monoclonal Antibody, researchers can investigate the expression levels and localization of the MYH7 protein in different cell types and tissues, gaining insights into its function and potential implications in muscle-related diseases.This monoclonal antibody offers high specificity and sensitivity in detecting the MYH7 protein, making it a valuable tool for studying muscle biology, cardiovascular diseases, and neuromuscular disorders. Its applications in research make it essential for scientists looking to advance our understanding of muscle function and the implications of MYH7 gene mutations in human health.
Product Name:
MYH7/β-MHC Monoclonal Antibody
SKU:
CAB22140
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1700-1800 of human MYH7/β-MHC (NP_000248.2).
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy.
Purification Method:
Affinity purification
Gene ID:
4625
Clone Number:
ARC55749
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using MYH7/β-MHC antibody (CAB22140) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 4s.
