The MYF5 Polyclonal Antibody (CAB16227) is a crucial tool for research involving MYF5, a key regulator of myogenesis and muscle development. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot and immunohistochemistry applications. By binding specifically to the MYF5 protein, this antibody allows for precise detection and analysis in various cell types, making it an ideal choice for studies in muscle biology, stem cell research, and regenerative medicine.
MYF5, a member of the myogenic regulatory factors family, plays a pivotal role in the determination and differentiation of muscle precursor cells. Its involvement in myogenesis makes it a significant target for investigating muscle disorders, muscle regeneration, and muscle-related diseases such as muscular dystrophy. Understanding the function and regulation of MYF5 is essential for developing therapeutic strategies aimed at promoting muscle repair and regeneration in various pathological conditions.
Product Name:
MYF5 Rabbit Polyclonal Antibody
SKU:
CAB16227
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 143-220 of human MYF5 (NP_005584.2).
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to contribute to E-box binding activity. Predicted to be involved in several processes, including muscle cell fate commitment; positive regulation of cell differentiation; and skeletal muscle cell differentiation. Predicted to act upstream of or within several processes, including animal organ development; regulation of cell-matrix adhesion; and somitogenesis. Located in nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
4617
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse testis, using MYF5 Rabbit pAb (CAB16227) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
