The MYCN Polyclonal Antibody (CAB0499) is a valuable tool for researchers studying MYCN, a transcription factor with important roles in cell growth and differentiation, particularly in the context of cancer. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards MYCN protein, allowing for accurate detection and analysis in various experimental settings.MYCN is a key player in the regulation of cell proliferation and survival, making it a target of interest in cancer research. Its overexpression is commonly associated with aggressive forms of neuroblastoma and other solid tumors, highlighting its potential as a therapeutic target. By utilizing the MYCN Polyclonal Antibody, researchers can gain insights into the mechanisms underlying MYCN-driven oncogenesis, paving the way for the development of novel targeted therapies.
Furthermore, this antibody has been validated for use in Western blot applications, ensuring reliable and reproducible results. Its versatility in detecting MYCN expression in different cell types makes it a valuable tool for investigating the role of MYCN in tumorigenesis and identifying potential therapeutic interventions. Researchers in the fields of oncology, molecular biology, and drug discovery will find the MYCN Polyclonal Antibody essential for their studies on MYCN-related pathways and associated diseases.
Product Name:
MYCN Rabbit Polyclonal Antibody
SKU:
CAB0499
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human N-Myc/MYCN (NP_005369.2).
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4613
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using N-Myc/MYCN Rabbit pAb (CAB0499) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
