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Myc-Tag Rabbit Monoclonal Antibody (CABE070)

SKU:
CABE070
$299
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Description

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Product Name:Myc-Tag Rabbit Monoclonal Antibody
SKU:CABE070
Size:100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Species independent
Immunogen:A synthetic peptide corresponding to Myc tag.
Sequence:EQKLISEEDL
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:10000 - 1:140000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:Myc; Myc tag; Myc-tag; Myc-Tag
Positive Sample:293T
Conjugate:Unconjugated
Observed MW:58kDa(NLK)/72kDa(YAP1)/55kDa(PTEN)

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Purification Method:Affinity purification
Clone Number:ARC5004-12
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Myc-Tag Rabbit mAb (AE070) at 1:120000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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