The MYC Polyclonal Antibody (CAB17332) is a valuable tool for researchers studying the MYC protein, a transcription factor with key roles in cell growth, proliferation, and apoptosis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.MYC is a critical regulator of cellular processes, and dysregulation of its expression is linked to various cancers and other diseases. By targeting the MYC protein, researchers can gain insights into its function and potential therapeutic implications in oncology and beyond.
This antibody provides a reliable means of detecting and analyzing MYC protein levels in different cell types, making it suitable for a range of research applications in molecular biology, cancer biology, and drug discovery. Researchers can use this tool to further their understanding of MYC's involvement in disease processes and potentially identify new targets for therapeutic intervention.
Product Name:
c-Myc Rabbit Polyclonal Antibody
SKU:
CAB17332
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 347-439 of human c-Myc (NP_001341799.1).
Sequence:
LRQI SNNR KCTS PRSS DTEE NVKR RTHN VLER QRRN ELKR SFFA LRDQ IPEL ENNE KAPK VVIL KKAT AYIL SVQA EEQK LISE EDLL RKRR E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
57kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using c-Myc antibody (CAB17332) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
