The Myc Polyclonal Antibody (CAB1309) is a vital tool for research involving the Myc protein, a transcription factor that plays a key role in cell proliferation, growth, and apoptosis. This antibody, generated in rabbits, is highly specific and sensitive for detection of Myc protein in human samples, making it ideal for a variety of research applications such as Western blotting. By binding to the Myc protein, this antibody enables accurate detection and analysis in different cell types, making it an essential tool for studies in cancer research, cell biology, and molecular biology.
Myc is a critical regulator of cell cycle progression and is frequently deregulated in cancer, making it a promising target for therapeutic intervention. Understanding the role of Myc in tumorigenesis is essential for developing targeted therapies that can selectively inhibit Myc-driven cancer cells. By utilizing the Myc Polyclonal Antibody (CAB1309), researchers can further investigate the function and regulation of the Myc protein, leading to potential advancements in cancer treatment and diagnosis.
Product Name:
c-Myc Rabbit Polyclonal Antibody
SKU:
CAB1309
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 347-439 of human c-Myc (NP_001341799.1).
Sequence:
LRQI SNNR KCTS PRSS DTEE NVKR RTHN VLER QRRN ELKR SFFA LRDQ IPEL ENNE KAPK VVIL KKAT AYIL SVQA EEQK LISE EDLL RKRR E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
MRTL; MYCC; c-Myc; bHLHe39
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus, nucleoplasm.
Calculated MW:
51kDa
Observed MW:
60kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method:
Affinity purification
Gene ID:
4609
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using c-Myc Rabbit pAb (CAB1309) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
