The Mus81 Polyclonal Antibody (CAB6818) is specifically designed for research involving Mus81, a key protein involved in DNA repair and genome stability. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Mus81 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.Mus81 is a crucial component in various DNA repair pathways, playing a vital role in maintaining genomic integrity and stability. Dysregulation of Mus81 has been associated with genetic disorders and cancer development, highlighting the importance of understanding its function in cellular processes.
Research on Mus81 can provide insights into mechanisms of DNA repair, replication, and genome maintenance, offering potential therapeutic targets for diseases related to genome instability.Overall, the Mus81 Polyclonal Antibody (CAB6818) serves as a valuable tool for investigating the role of Mus81 in DNA repair pathways, genome stability, and cancer development. Its high specificity and sensitivity make it an ideal choice for researchers studying the molecular mechanisms underlying genetic disorders and cancer biology.
Product Name:
MUS81 Rabbit Polyclonal Antibody
SKU:
CAB6818
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3).
This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.
Purification Method:
Affinity purification
Gene ID:
80198
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MUS81 Rabbit pAb (CAB6818) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
