The MTRF1 Polyclonal Antibody (PAC049786) is a valuable tool for research involving MTRF1, a protein involved in mitochondrial translation termination. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the MTRF1 protein, researchers can detect and analyze its expression in various cell types, making it ideal for studies in mitochondrial biology and related fields.MTRF1 is a key player in the process of mitochondrial protein synthesis and termination, making it a crucial component in understanding mitochondrial function and dysfunction.
Research on MTRF1 has implications for various diseases, including mitochondrial disorders, neurodegenerative diseases, and metabolic disorders. By studying the role of MTRF1, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
Immunohistochemistry of paraffin-embedded human liver cancer using PACO49786 at dilution of 1:100.
Immunofluorescence staining of Hela cells with PACO49786 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: MTRF1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 55 kDa. Observed band size: 53 kDa.
Background:
Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.
