mTOR Polyclonal Antibody (CAB24743)
- SKU:
- CAB24743
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | mTOR Polyclonal Antibody |
| SKU: | CAB24743 |
| Size: | 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human mTOR (NP_004949.1). |
| Sequence: | CHTV MEVL REHK DSVM AVLE AFVY DPLL NWRL MDTN TKGN KRSR TRTD SYSA GQSV EILD GVEL GEPA HKKT GTTV PESI HSFI GDGL VKPE ALNK KAIQ I |
| Tested Applications: | WB |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR |
| Positive Sample: | Hep G2 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein. |
| Calculated MW: | 289kDa |
| Observed MW: | 289kDa |
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 2475 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Hep G2 cells using mTOR Rabbit pAb(CAB24743) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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