The MTIF2 Polyclonal Antibody (CAB16402) is a valuable tool for researchers studying MTIF2, a protein involved in mitochondrial protein synthesis. This antibody, produced in rabbits, has high reactivity with human samples and is suitable for Western blot applications. By binding specifically to MTIF2, this antibody allows for the detection and analysis of this protein in various cell types, making it an important asset for research in molecular biology and mitochondrial function.
MTIF2 is a key player in the process of mitochondrial translation initiation, a crucial step in protein synthesis within the mitochondria. Understanding the role of MTIF2 is essential for unraveling the complexities of mitochondrial biology and its implications in various diseases such as mitochondrial disorders and cancer. By targeting MTIF2, researchers can gain insights into the mechanisms underlying these conditions and potentially develop new therapeutic strategies.
Product Name:
MTIF2 Rabbit Polyclonal Antibody
SKU:
CAB16402
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2).
During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins.
Purification Method:
Affinity purification
Gene ID:
4528
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MTIF2 Rabbit pAb (CAB16402) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
