The MTHFD2 Polyclonal Antibody (CAB10386) is a valuable tool for researchers studying MTHFD2, a mitochondrial enzyme involved in one-carbon metabolism and folate cycle regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MTHFD2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in biochemistry, metabolism, and cancer research.
MTHFD2 plays a crucial role in nucleotide synthesis and DNA methylation, making it a key player in cell proliferation and survival. Dysregulation of MTHFD2 has been linked to various diseases, including cancer and neurological disorders, highlighting its potential as a therapeutic target. By studying the function and regulation of MTHFD2, researchers can gain insights into its role in disease pathology and potentially identify novel treatment strategies.
Product Name:
MTHFD2 Rabbit Polyclonal Antibody
SKU:
CAB10386
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Purification Method:
Affinity purification
Gene ID:
10797
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MTHFD2 Rabbit pAb (CAB10386) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
