The MTHFD2 Monoclonal Antibody is a valuable tool for researchers studying MTHFD2, a key enzyme involved in folate metabolism and cell proliferation. This monoclonal antibody, generated in mice, shows high specificity and sensitivity for detecting MTHFD2 in various cell types and tissues. It has been validated for use in applications such as immunohistochemistry and flow cytometry.MTHFD2 is a mitochondrial enzyme that plays a crucial role in one-carbon metabolism, which is essential for nucleotide biosynthesis and DNA methylation.
Aberrant expression or activity of MTHFD2 has been linked to various cancers and other diseases, making it an attractive target for therapeutic intervention. By utilizing the MTHFD2 Monoclonal Antibody, researchers can gain valuable insights into the function and regulation of this enzyme, advancing our understanding of its role in health and disease.
Product Name:
MTHFD2 Monoclonal Antibody
SKU:
CAB22653
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant funsion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Purification Method:
Affinity purification
Gene ID:
10797
Clone Number:
ARC58228
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using MTHFD2 Rabbit mAb (CAB22653) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
