The MTHFD1 Polyclonal Antibody (CAB8661) is a vital tool for researchers studying MTHFD1, an enzyme involved in folate metabolism and DNA synthesis. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the MTHFD1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in fields such as cancer research and developmental biology.
MTHFD1, also known as methylenetetrahydrofolate dehydrogenase 1, plays a crucial role in providing one-carbon units for purine and thymidylate biosynthesis. Its involvement in these essential cellular processes makes it a key target for research into diseases such as cancer and neural tube defects. Further understanding of MTHFD1 function is essential for developing targeted therapies that aim to manipulate folate metabolism in diseases where it is dysregulated.
Product Name:
MTHFD1 Rabbit Polyclonal Antibody
SKU:
CAB8661
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.
Purification Method:
Affinity purification
Gene ID:
4522
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MTHFD1 antibody (CAB8661) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
