The MTH1 Rabbit Monoclonal Antibody (CAB3938) is a powerful tool for researchers studying MTH1, an enzyme that plays a key role in protecting cells from oxidative damage and maintaining genomic stability. This antibody, developed using rabbit monoclonal technology, is highly specific to human MTH1 and has been validated for use in various applications including Western blotting.MTH1 is a critical enzyme in the base excision repair pathway, where it acts to sanitize oxidized nucleotides before they can be incorporated into DNA, thereby preventing mutations. Dysregulation of MTH1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and aging-related conditions.
By targeting MTH1, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.This MTH1 Rabbit Monoclonal Antibody provides researchers with a reliable tool for detecting MTH1 protein levels in different cell types and tissues, allowing for in-depth analysis of its role in cellular processes. Its high specificity and sensitivity make it an ideal choice for studies in cancer biology, aging research, and drug discovery efforts aimed at targeting MTH1 for therapeutic purposes. Unlock the potential of MTH1 research with this cutting-edge antibody.
Product Name:
MTH1 Rabbit Monoclonal Antibody
SKU:
CAB3938
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-200 of human MTH1 (P36639).
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
Purification Method:
Affinity purification
Gene ID:
4521
Clone Number:
ARC0869
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MTH1 Rabbit mAb (CAB3938) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
