The MTA2 Polyclonal Antibody (CAB2243) is a crucial tool for researchers studying the MTA2 protein, a component of the nucleosome remodeling and deacetylase (NuRD) complex that plays a key role in gene regulation and chromatin remodeling. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.MTA2 is involved in important cellular processes such as transcriptional regulation, DNA repair, and cell differentiation.
Dysregulation of MTA2 expression has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions. The MTA2 Polyclonal Antibody enables precise detection and analysis of MTA2 protein levels in different cell types, providing valuable insights for studies in molecular biology, oncology, and other fields of research.
Product Name:
[KO Validated] MTA2 Rabbit Polyclonal Antibody
SKU:
CAB2243
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human MTA2 (NP_004730.2).
WB,1:500 - 1:1000 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
PID; MTA1L1; A2
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
75kDa
Observed MW:
75kDa
This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.
Purification Method:
Affinity purification
Gene ID:
9219
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and MTA2 knockout (KO) 293T cells, using [KO Validated] MTA2 Rabbit pAb (CAB2243) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
