The MT-ND6 Polyclonal Antibody (CAB17991) is a valuable tool for research involving the MT-ND6 protein, a key component of the mitochondrial respiratory chain. This antibody, derived from rabbit sources, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the MT-ND6 protein, researchers can detect and analyze this important mitochondrial protein in various cell types, making it ideal for studies in bioenergetics, mitochondrial function, and diseases related to mitochondrial dysfunction.MT-ND6 is a subunit of NADH dehydrogenase, an enzyme complex essential for the production of ATP through oxidative phosphorylation in the mitochondria.
Dysfunction in the mitochondrial respiratory chain, including mutations in the MT-ND6 gene, has been implicated in various human diseases such as mitochondrial myopathy, Leber's hereditary optic neuropathy, and other mitochondrial disorders. Therefore, understanding the role and regulation of MT-ND6 is crucial for advancing research in mitochondrial biology and developing potential therapies for these debilitating conditions.
Product Name:
MT-ND6 Rabbit Polyclonal Antibody
SKU:
CAB17991
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 80-174 of human MT-ND6 (YP_003024037.1).
mitochondrial inner membrane, mitochondrial respiratory chain complex I
Calculated MW:
19kDa
Observed MW:
24kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Predicted to be located in mitochondrial inner membrane. Implicated in Leber hereditary optic neuropathy; Leigh disease; and spinal muscular atrophy with lower extremity predominante 2B.
Purification Method:
Affinity purification
Gene ID:
4541
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using MT-ND6 Rabbit pAb (CAB17991) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
