The MT-ND5 Polyclonal Antibody (CAB17972) is a valuable tool for researchers studying mitochondrial complex I, specifically targeting the MT-ND5 protein. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.The MT-ND5 protein is a subunit of mitochondrial complex I, which plays a crucial role in the electron transport chain and ATP production within the mitochondria. Dysregulation of complex I function has been implicated in various human diseases, including neurodegenerative disorders and cancer.
By detecting and analyzing the MT-ND5 protein in different cell types, the MT-ND5 Polyclonal Antibody enables researchers to unravel the role of mitochondrial complex I in health and disease. Its application in immunohistochemistry allows for localization of the protein within tissues, providing valuable insights into mitochondrial function in various physiological and pathological conditions. This antibody is a valuable tool for advancing research in bioenergetics, mitochondrial biology, and related fields.
Product Name:
MT-ND5 Rabbit Polyclonal Antibody
SKU:
CAB17972
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human MT-ND5 (YP_003024036.1).
mitochondrial inner membrane, mitochondrial respiratory chain complex I
Calculated MW:
67kDa
Observed MW:
70kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and MELAS syndrome.
Purification Method:
Affinity purification
Gene ID:
4540
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MT-ND5 Rabbit pAb (CAB17972) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
