The MT-ND4 Polyclonal Antibody (CAB17970) is a research tool designed for the detection and analysis of the MT-ND4 protein, a subunit of the mitochondrial respiratory chain complex I. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MT-ND4 protein, this antibody allows for the investigation of mitochondrial function and dysfunction in various cell types, making it well-suited for studies in bioenergetics, metabolism, and mitochondrial-related diseases.
MT-ND4 is a key component of the electron transport chain in mitochondria, playing a crucial role in ATP production and cellular energy metabolism. Dysregulation of MT-ND4 function has been linked to a variety of diseases, including mitochondrial disorders, neurodegenerative diseases, and cancer. Research into the function and regulation of MT-ND4 is essential for understanding the implications of mitochondrial dysfunction and developing potential therapeutic strategies for mitochondrial-related disorders.
Product Name:
MT-ND4 Rabbit Polyclonal Antibody
SKU:
CAB17970
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 401-459 of human MT-ND4 (YP_002124311.1).
mitochondrial inner membrane, mitochondrial respiratory chain complex I
Calculated MW:
52kDa
Observed MW:
51kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Parkinson's disease; macular degeneration; and schizophrenia. Biomarker of Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
4538
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using MT-ND4 Rabbit pAb (CAB17970) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
