The MT-ND3 Polyclonal Antibody (CAB17969) is a valuable tool for researchers studying the mitochondrial gene MT-ND3, which encodes a subunit of the NADH dehydrogenase complex. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MT-ND3 protein, this antibody enables researchers to detect and analyze MT-ND3 expression in various cell types, making it an ideal choice for studies in mitochondrial biology and disease research.The MT-ND3 protein plays a crucial role in the electron transport chain, where it participates in the generation of ATP, the energy currency of the cell.
Dysregulation of MT-ND3 has been implicated in a variety of diseases, including mitochondrial disorders and neurodegenerative conditions. Therefore, understanding the function and expression of MT-ND3 is essential for unraveling the mechanisms of these diseases and developing potential therapeutic strategies.Overall, the MT-ND3 Polyclonal Antibody (CAB17969) offers researchers a reliable tool for investigating the role of MT-ND3 in cellular metabolism and disease pathogenesis, paving the way for advancements in mitochondrial research.
Product Name:
MT-ND3 Rabbit Polyclonal Antibody
SKU:
CAB17969
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-70 of human MT-ND3 (YP_003024033.1).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MTND3; ND3; MT-ND3
Positive Sample:
293T,PC-3,Mouse heart,Mouse brain
Cellular Localization:
mitochondrial inner membrane, mitochondrial respiratory chain complex I
Calculated MW:
13kDa
Observed MW:
15kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and Parkinson's disease.
Purification Method:
Affinity purification
Gene ID:
4537
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MT-ND3 Rabbit pAb (CAB17969) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
