The MT-CO2 Polyclonal Antibody (CAB11154) is a specialized research tool designed for studying the MT-CO2 protein, a subunit of cytochrome c oxidase, also known as Complex IV of the mitochondrial electron transport chain. This antibody is produced in rabbits and exhibits high reactivity specifically with human samples, making it a valuable tool for researchers studying mitochondrial function and oxidative phosphorylation.The MT-CO2 Polyclonal Antibody is validated for use in Western blot applications, where it binds specifically to the MT-CO2 protein, allowing for accurate detection and analysis in various cell types and tissues. This antibody is particularly useful for researchers working in the fields of biochemistry, cell biology, and molecular biology, as it enables the investigation of mitochondrial function and energy production processes.
The MT-CO2 protein plays a crucial role in the electron transport chain, which is responsible for generating the majority of ATP, the cell's primary energy source. Dysregulation of mitochondrial function, including mutations in MT-CO2, has been linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer. By using the MT-CO2 Polyclonal Antibody, researchers can gain insights into the role of this protein in health and disease, potentially leading to the development of novel therapeutic strategies targeting mitochondrial dysfunction.
Antibody Name:
Anti-MTCO2 Antibody
Antibody SKU:
CAB11154
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse MTCO2 (NP_904331.1).
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
Mouse liver, Mouse heart, Mouse kidney, Rat liver, Rat kidney
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse MTCO2 (NP_904331.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:
25kDa
Observed MW:
24kDa
Synonyms:
MT-CO2, COII, MTCO2, COX2
Background:
Western blot analysis of extracts of various cell lines, using MTCO2 antibody (CAB11154) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Immunofluorescence analysis of U2OS cells using MTCO2 antibody (CAB11154) at dilution of 1:100. Blue: DAPI for nuclear staining.
