The MSH6 Polyclonal Antibody (CAB3177) is a valuable tool for researchers studying MSH6, a key protein involved in DNA mismatch repair. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MSH6 protein, this antibody enables the detection and analysis of MSH6 in various cell types, making it an essential reagent for studies in genetics and cancer research.MSH6 is a crucial component of the DNA mismatch repair system, which helps maintain genomic stability and prevent mutations that can lead to cancer development.
Mutations in MSH6 have been linked to hereditary nonpolyposis colorectal cancer (HNPCC), also known as Lynch syndrome, highlighting the importance of understanding its role in DNA repair mechanisms. Research on MSH6 using this antibody may provide insights into cancer development and help guide the development of targeted therapies for individuals with MSH6-related cancers.
Product Name:
MSH6 Rabbit Polyclonal Antibody
SKU:
CAB3177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1360 of human MSH6 (P52701).
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
2956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MSH6 Rabbit pAb (CAB3177) at 1:300 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
