The MSH2 Polyclonal Antibody (CAB21494) is a valuable tool for researchers studying MSH2, a key protein involved in DNA mismatch repair. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MSH2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for investigations in genetics, oncology, and other fields where DNA repair mechanisms are of interest.MSH2 is a crucial component of the DNA mismatch repair system, responsible for correcting errors that occur during DNA replication. Mutations in the MSH2 gene are associated with Lynch syndrome, a hereditary cancer predisposition syndrome, highlighting the importance of studying this protein in the context of cancer research.
Additionally, MSH2 plays a role in maintaining genomic stability and preventing the accumulation of mutations that can lead to various diseases.By utilizing the MSH2 Polyclonal Antibody, researchers can gain valuable insights into the function of MSH2 and its impact on cellular processes. This antibody is a versatile tool for investigating DNA repair pathways, genetic instability, and the molecular mechanisms underlying cancer development. Its specificity and reliability make it an essential reagent for any laboratory working to unravel the complexities of DNA repair and its implications for human health.
Product Name:
MSH2 Polyclonal Antibody
SKU:
CAB21494
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4436
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using [KO Validated] MSH2 Rabbit pAb (CAB21494) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
