Mouse REN (Renin) ELISA Kit - High Sensitivity
- SKU:
- AEES00622
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 4.52 pg/mL
- Range:
- 7.81-500 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse REN (Renin) ELISA Kit - High Sensitivity
The Mouse REN (Renin) ELISA Kit - High Sensitivity is a specialized assay meticulously crafted to quantitatively measure Renin levels in various mouse biological samples with outstanding precision and sensitivity.
Renin, an essential enzyme of the renin-angiotensin system, plays a pivotal role in regulating blood pressure and fluid-electrolyte balance by initiating a cascade of biochemical reactions that ultimately lead to the production of angiotensin peptides involved in cardiovascular homeostasis. This high-sensitivity ELISA kit empowers researchers to explore the intricate role of Renin in blood pressure regulation, vascular function, and electrolyte balance in mouse models. Accurate quantification of Renin levels is crucial for unraveling the complex mechanisms underlying hypertension, cardiovascular diseases, and renal disorders, providing valuable insights into the pathophysiology of cardiovascular health and kidney function. Manufactured under rigorous quality control standards, the Mouse REN (Renin) ELISA Kit - High Sensitivity ensures robust and reliable results, making it an indispensable tool for cutting-edge research in hypertension, cardiovascular biology, and vascular health. Whether investigating Renin's involvement in blood pressure regulation or studying cardiovascular disorders, this ELISA kit provides researchers with a precise and sensitive method for exploring the vital role of Renin in cardiovascular physiology and pathology.
Product Name: | Mouse REN (Renin) ELISA Kit - High Sensitivity |
Product Code: | AEES00622 |
Assay Type: | Sandwich |
Format: | 96 Assays |
Assay Time: | 3.5h |
Reactivity: | Mouse |
Detection Range: | 7.81-500 pg/mL |
Sensitivity: | 4.52 pg/mL |
Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
Specificity: | This kit recognizes Mouse REN in samples. No significant cross-reactivity or interference between Mouse REN and analogues was observed. |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse REN concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse REN. You can calculate the concentration of Mouse REN in the samples by comparing the OD of the samples to the standard curve.
Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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