Mouse LEPR (Leptin Receptor) ELISA Kit - High Sensitivity
- SKU:
- AEES00621
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 3.75 ng/mL
- Range:
- 6.25-400 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse LEPR (Leptin Receptor) ELISA Kit - High Sensitivity
The Mouse LEPR (Leptin Receptor) ELISA Kit - High Sensitivity is a specialized assay designed to quantitatively measure Leptin Receptor levels in various mouse biological samples with exceptional accuracy and sensitivity.
Leptin Receptors, integral to the body's energy homeostasis and metabolic regulation, play a pivotal role in mediating the effects of Leptin, the key hormone regulating appetite and metabolism. This high-sensitivity ELISA kit offers researchers a precise tool to explore the intricate pathways governed by Leptin Receptors, shedding light on their involvement in metabolic signaling, energy balance, and body weight regulation in mouse models. Accurate quantification of Leptin Receptor levels is crucial for unraveling the mechanisms underlying metabolic disorders, obesity, and metabolic syndromes. With its stringent quality control standards, the Mouse LEPR (Leptin Receptor) ELISA Kit - High Sensitivity by Assay Genie ensures robust and reproducible results, empowering researchers to delve deeper into the role of Leptin Receptors in metabolic health. Whether investigating Leptin signaling pathways or studying metabolic diseases, this ELISA kit is an invaluable resource for cutting-edge research in metabolic biology and endocrinology.
| Product Name: | Mouse LEPR (Leptin Receptor) ELISA Kit - High Sensitivity |
| Product Code: | AEES00621 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 6.25-400 ng/mL |
| Sensitivity: | 3.75 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
| Specificity: | This kit recognizes Mouse LEPR in samples. No significant cross-reactivity or interference between Mouse LEPR and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse LEPR concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEPR. You can calculate the concentration of Mouse LEPR in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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