Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit - High Sensitivity
- SKU:
- AEES00596
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit - High Sensitivity
**** The Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted to facilitate the accurate quantification of Kidney Injury Molecule 1 (KIM-1) levels in various biological samples derived from mouse models. KIM-1 is a critical biomarker associated with renal injury and renal pathology, pivotal for the detection and assessment of kidney damage and function, making it a vital target for research into kidney diseases and renal health. Developed with a focus on high sensitivity, this ELISA kit offers researchers a precise tool to delve into the intricate mechanisms of kidney injury, renal function, and related pathologies in mouse models with exceptional accuracy.
Through the high-sensitivity detection capabilities of this kit, researchers can gain valuable insights into the role of KIM-1 in kidney injury, inflammation, and disease progression. Ensuring reliable and reproducible results, the Mouse KIM-1 ELISA Kit - High Sensitivity from Assay Genie upholds stringent quality control standards to deliver robust performance. Easy to use and meticulously designed, this kit provides an excellent choice for researchers investigating kidney injury, renal function, and the pathophysiology of kidney diseases in mouse models, enabling advancements in renal research and therapeutic strategies.
Product Name: | Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit - High Sensitivity |
Product Code: | AEES00596 |
Assay Type: | Sandwich |
Format: | 96T |
Assay Time: | 3.5h |
Reactivity: | Mouse |
Detection Range: | 31.25-2000 pg/mL |
Sensitivity: | 18.75 pg/mL |
Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
Specificity: | This kit recognizes Mouse KIM-1 in samples. No significant cross-reactivity or interference between Mouse KIM-1 and analogues was observed. |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse KIM-1 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse KIM-1. You can calculate the concentration of Mouse KIM-1 in the samples by comparing the OD of the samples to the standard curve.
Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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