Mouse IL-17A (Interleukin 17A) ELISA Kit - High Sensitivity
- SKU:
- AEES00593
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 2.19 pg/mL
- Range:
- 7.81-500 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse IL-17A ELISA Kit - High Sensitivity
**** The Mouse IL-17A ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously crafted to quantitatively measure IL-17A levels in diverse biological samples sourced from mice.
IL-17A, a pivotal pro-inflammatory cytokine, serves as a key regulator of immune responses, particularly in the context of promoting inflammation and autoimmune reactions. Its multifaceted roles encompass modulating various cellular functions, immune cell activities, and disease pathogenesis across a spectrum of inflammatory and autoimmune conditions. With a high level of sensitivity, this specialized ELISA kit offers researchers the precise tools needed to investigate the intricate functions and regulatory roles of IL-17A in immune-mediated processes and inflammatory disorders within mouse models. By providing exceptional accuracy and detection capabilities, this high-sensitivity kit empowers researchers to elucidate the complex mechanisms underlying the involvement of IL-17A in immune responses and the development of inflammatory diseases. Maintaining a focus on quality and performance, the Mouse IL-17A ELISA Kit - High Sensitivity from Assay Genie ensures reliable and reproducible results, enabling researchers to conduct in-depth studies with confidence. Designed for ease of use and robust performance, this kit is an excellent choice for exploring IL-17A biology and immune-related research applications in mouse models.
Product Name: | Mouse IL-17A (Interleukin 17A) ELISA Kit - High Sensitivity |
Product Code: | AEES00593 |
Assay Type: | Sandwich |
Format: | 96T |
Assay Time: | 3.5h |
Reactivity: | Mouse |
Detection Range: | 7.81-500 pg/mL |
Sensitivity: | 2.19 pg/mL |
Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
Specificity: | This kit recognizes Mouse IL-17A in samples. No significant cross-reactivity or interference between Mouse IL-17A and analogues was observed. |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-17A concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-17A. You can calculate the concentration of Mouse IL-17A in the samples by comparing the OD of the samples to the standard curve.
Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|