Mouse IL-1α (Interleukin 1Alpha) ELISA Kit - High Sensitivity
- SKU:
- AEES00626
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 3.01 pg/mL
- Range:
- 7.81-500 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse IL-1α ELISA Kit - High Sensitivity
The Mouse IL-1α ELISA Kit - High Sensitivity is a specialized assay developed for the accurate and sensitive quantification of Interleukin-1 alpha (IL-1α) levels in various mouse biological samples. IL-1α is a pivotal pro-inflammatory cytokine that participates in a diverse range of cellular processes, including cell growth, differentiation, and programmed cell death. This cytokine exerts critical functions in initiating and regulating immune responses and inflammatory reactions, contributing significantly to the body's defense mechanisms and the pathogenesis of various inflammatory conditions, autoimmune disorders, and infectious diseases. With its high sensitivity and specificity, the Mouse IL-1α ELISA Kit enables researchers to precisely measure IL-1α concentrations, providing vital insights into its roles in disease mechanisms and as a potential therapeutic target.
Accurate quantification of IL-1α levels is crucial for elucidating the complex pathways involved in immune responses and inflammatory conditions, paving the way for the development of targeted therapeutic interventions. Backed by stringent quality control measures, this ELISA kit from Assay Genie offers robust performance, delivering reliable and reproducible results for research applications. Its user-friendly design and exceptional performance make it an invaluable tool for investigators studying inflammation, immunology, and disease pathogenesis. Get ready to uncover the intricate roles of IL-1α in biological processes with confidence and precision using the Mouse IL-1α ELISA Kit - High Sensitivity.
| Product Name: | Mouse IL-1α (Interleukin 1Alpha) ELISA Kit - High Sensitivity |
| Product Code: | AEES00626 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 3.01 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
| Specificity: | This kit recognizes Mouse IL-1α in samples. No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1α concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
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