Mouse IFN-γ (Interferon Gamma) ELISA Kit - High Sensitivity
- SKU:
- AEES00594
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 4.53 pg/mL
- Range:
- 7.81-500 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse IFN-γ (Interferon Gamma) ELISA Kit - High Sensitivity
**** The Mouse IFN-γ (Interferon Gamma) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously developed for the precise quantitative measurement of Interferon Gamma (IFN-γ) levels within a variety of biological samples sourced from mice.
IFN-γ, a pivotal cytokine known for its essential role in modulating immune responses, has diverse functions in regulating immune surveillance, cellular differentiation, and inflammatory processes. It serves as a critical mediator in orchestrating immune defenses against infections, tumors, and autoimmune conditions through its intricate signaling pathways. Equipped with high sensitivity, this specialized ELISA kit empowers researchers to explore the intricate roles and diverse functions of IFN-γ in immune regulation and host defense mechanisms within mouse models. By delivering exceptional accuracy and detection capabilities, this high-sensitivity kit provides a valuable tool for investigating the involvement of IFN-γ in immune responses, inflammation, and disease pathogenesis. Focused on quality and performance, the Mouse IFN-γ ELISA Kit - High Sensitivity from Assay Genie ensures dependable and reproducible results, facilitating detailed investigations into the roles of IFN-γ in immune-related processes and disease models. Designed for user-friendly operation and robust performance, this kit is an excellent choice for researchers conducting studies in the fields of immunology and inflammatory diseases using mouse models.
| Product Name: | Mouse IFN-γ (Interferon Gamma) ELISA Kit - High Sensitivity |
| Product Code: | AEES00594 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 4.53 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
| Specificity: | This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
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