Mouse ENG (Endoglin) ELISA Kit - High Sensitivity
- SKU:
- AEES00615
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 0.47 ng/mL
- Range:
- 0.78-50 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Mouse ENG (Endoglin) ELISA Kit - High Sensitivity
The Mouse ENG (Endoglin) ELISA Kit - High Sensitivity is a specialized research tool designed to quantitatively measure the levels of endoglin in various biological samples from mouse models. Endoglin, also known as CD105, is a key protein involved in endothelial cell function, angiogenesis, and regulation of vascular homeostasis.
With high sensitivity, this ELISA kit enables researchers to accurately quantify endoglin, providing valuable insights into its roles in vascular development, angiogenesis, and physiological or pathological processes affecting the endothelium in murine models. Precise measurement of endoglin levels is crucial for understanding its contribution to vascular health and disease progression. Manufactured under rigorous quality control standards, the Mouse ENG ELISA Kit - High Sensitivity offered by Assay Genie ensures reliable and reproducible results, making it an excellent choice for research studies requiring robust performance and ease of use in investigating endoglin-related pathways and functions.
| Product Name: | Mouse ENG (Endoglin) ELISA Kit - High Sensitivity |
| Product Code: | AEES00615 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.78-50 ng/mL |
| Sensitivity: | 0.47 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25μL |
| Specificity: | This kit recognizes Mouse ENG in samples. No significant cross-reactivity or interference between Mouse ENG and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse ENG concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ENG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ENG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ENG. You can calculate the concentration of Mouse ENG in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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