The MRPS6 Polyclonal Antibody (CAB9884) is a valuable tool for researchers studying MRPS6, a protein involved in mitochondrial ribosome assembly and translation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the MRPS6 protein, this antibody enables researchers to detect and analyze MRPS6 expression in a variety of cell types.MRPS6 is a crucial component of the mitochondrial ribosome and plays a key role in protein synthesis within the mitochondria.
Research on MRPS6 is important for understanding mitochondrial function and its implications in various diseases, including metabolic disorders and cancer. By investigating the function of MRPS6, researchers can gain insights into mitochondrial dysfunction and its role in disease progression.Overall, the MRPS6 Polyclonal Antibody is a valuable tool for researchers studying mitochondrial biology and protein synthesis, providing a means to investigate the role of MRPS6 in various cellular processes and disease states.
Product Name:
MRPS6 Rabbit Polyclonal Antibody
SKU:
CAB9884
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human MRPS6 (NP_115865.1).
B cells,MCF7,22Rv1,Jurkat,Mouse kidney,Mouse heart
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
14kDa
Observed MW:
14kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S6P family. Pseudogenes corresponding to this gene are found on chromosomes 1p and 12q.
Purification Method:
Affinity purification
Gene ID:
64968
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MRPS6 antibody (CAB9884) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
