The MRPS35 Polyclonal Antibody (CAB15878) is a valuable tool for research involving MRPS35, a mitochondrial ribosomal protein essential for protein synthesis in the mitochondria. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications. It specifically binds to the MRPS35 protein, allowing for detection and analysis in various cell types and tissues.MRPS35 plays a vital role in mitochondrial function and has been implicated in various diseases, including cancer and neurodegenerative disorders.
Research into the function and regulation of MRPS35 is crucial for understanding mitochondrial protein synthesis and its role in disease development. The MRPS35 Polyclonal Antibody is an invaluable tool for investigating the function of MRPS35 in health and disease, making it a valuable asset for studies in cell biology and mitochondrial research.
Product Name:
MRPS35 Rabbit Polyclonal Antibody
SKU:
CAB15878
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human MRPS35 (NP_068593.2).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.
Purification Method:
Affinity purification
Gene ID:
60488
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS35 Rabbit pAb (CAB15878) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
