The MRPS34 Polyclonal Antibody (Paco22420) is a powerful tool for researchers studying mitochondrial ribosomal protein S34 (MRPS34), a key player in mitochondrial function and protein synthesis. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously validated for use in Western blot applications.MRPS34 is a crucial component of the mitochondrial ribosome, essential for the translation of mitochondrial DNA-encoded proteins. Dysregulation of MRPS34 has been linked to various diseases, including mitochondrial disorders and metabolic syndromes.
By targeting MRPS34 with this antibody, researchers can effectively study its role in mitochondrial biology and its implications in disease pathology.With its high reactivity and specificity, the MRPS34 Polyclonal Antibody (Paco22420) is an invaluable tool for investigators in the fields of biochemistry, cell biology, and genetics. By enabling the detection and analysis of MRPS34 in various cell types, this antibody opens up new avenues for understanding the complex mechanisms underlying mitochondrial function and dysfunction.
Antibody Name:
MRPS34 Antibody (PACO22420)
Antibody SKU:
PACO22420
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from C-terminal of human MRPS34.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, HUVEC cells, HT-29 cells, A549 cells and Jurkat cells, using MRPS34 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants.
Synonyms:
mitochondrial 28S ribosomal protein S34; mitochondrial ribosomal protein S34; MRP-S12; MRP-S34; RT34
UniProt Protein Function:
MRPS34: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.Chromosomal Location of Human Ortholog: 16p13.3Cellular Component: mitochondrial inner membrane; mitochondrionBiological Process: mitochondrial translation
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
