The MRPS33 Polyclonal Antibody (CAB14893) is a valuable tool for researchers studying mitochondrial ribosomal protein S33 (MRPS33), a key component of the mitochondrial ribosome. This antibody, produced through immunization of rabbits, is highly specific to human samples and is validated for use in Western blot applications.MRPS33 is essential for protein synthesis within the mitochondria, making it crucial for mitochondrial function and cellular metabolism. Dysregulation of MRPS33 has been implicated in various diseases, including metabolic disorders and cancer.
This antibody allows for the detection and analysis of MRPS33 in different cell types, making it ideal for investigations into mitochondrial biology and disease mechanisms.By targeting MRPS33, researchers can gain insights into mitochondrial protein synthesis, mitochondrial function, and the role of MRPS33 in disease development. This antibody is a valuable tool for studies in mitochondrial biology, metabolic disorders, and cancer research, offering a deeper understanding of cellular processes and potential therapeutic targets.
Product Name:
MRPS33 Rabbit Polyclonal Antibody
SKU:
CAB14893
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human MRPS33 (NP_057155.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q
Purification Method:
Affinity purification
Gene ID:
51650
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS33 Rabbit pAb (CAB14893) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
