The MRPS31 Polyclonal Antibody (CAB12923) is a valuable tool for researchers studying MRPS31, a mitochondrial ribosomal protein involved in protein synthesis within the mitochondria. The antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the MRPS31 protein, allowing for accurate detection and analysis in various cell types.MRPS31 is essential for maintaining mitochondrial function and has been linked to cellular processes such as oxidative phosphorylation and apoptosis. Dysregulation of MRPS31 has been associated with diseases such as cancer and neurodegenerative disorders, making it a promising target for therapeutic development.
Understanding the role of MRPS31 in these conditions can provide valuable insights into disease mechanisms and potential treatment strategies.The MRPS31 Polyclonal Antibody is a reliable tool for researchers interested in the molecular functions and pathological implications of MRPS31. Its specificity and sensitivity make it an ideal choice for studies in mitochondrial biology, cancer research, and other fields related to protein synthesis and cellular metabolism.
Product Name:
MRPS31 Rabbit Polyclonal Antibody
SKU:
CAB12923
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 66-395 of human MRPS31 (NP_005821.2).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13.
Purification Method:
Affinity purification
Gene ID:
10240
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS31 Rabbit pAb (CAB12923) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
