The MRPS30 Polyclonal Antibody (CAB5841) is a powerful tool for researchers studying the mitochondrial ribosomal protein S30 (MRPS30). This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the MRPS30 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in mitochondrial biology and related fields.MRPS30 is a key component of the mitochondrial ribosome, playing a crucial role in protein synthesis within the mitochondria. Dysfunction of MRPS30 has been implicated in various diseases, including cancer and mitochondrial disorders.
By understanding the function and regulation of MRPS30, researchers can uncover potential therapeutic targets for these conditions and further explore the role of mitochondrial protein synthesis in overall cellular health.Overall, the MRPS30 Polyclonal Antibody (CAB5841) is a valuable tool for researchers looking to delve into the intricate mechanisms of mitochondrial protein synthesis and its implications in disease pathology. Its high specificity and reliability make it an indispensable resource for advancing our understanding of mitochondrial biology.
Product Name:
MRPS30 Rabbit Polyclonal Antibody
SKU:
CAB5841
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PAP; PDCD9; S30mt; MRP-S30; MRPS30
Positive Sample:
SW620,HL-60,HeLa,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
50kDa
Observed MW:
54kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.
Purification Method:
Affinity purification
Gene ID:
10884
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS30 Rabbit pAb (CAB5841) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
