The MRPS18C Polyclonal Antibody (CAB18453) is a valuable tool for researchers studying MRPS18C, an essential protein in the mitochondrial ribosome involved in protein synthesis. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples and has been validated for use in Western blot applications. By targeting the MRPS18C protein, researchers can investigate its role in mitochondrial function and its implications in various cellular processes.MRPS18C is a key component of the mitochondrial ribosome, where it assists in the translation of mitochondrial DNA into functional proteins necessary for energy production.
Dysfunction in MRPS18C has been associated with mitochondrial disorders and metabolic diseases, highlighting its importance in maintaining cellular homeostasis. Researchers studying mitochondrial biology, metabolism, and genetic disorders will benefit from using this antibody to elucidate the function of MRPS18C in health and disease.
Product Name:
MRPS18C Rabbit Polyclonal Antibody
SKU:
CAB18453
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human MRPS18C (NP_057151.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 8p, 12p, 15q, and 22q.
Purification Method:
Affinity purification
Gene ID:
51023
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS18C Rabbit pAb (CAB18453) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
