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Anti-MRPS16 Antibody (CAB9874)

SKU:
CAB9874
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-MRPS16 Antibody CAB9874
  • Cell Biology Antibodies 12 Anti-MRPS16 Antibody CAB9874
€139 - €419
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Description

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Anti-MRPS16 Antibody (CAB9874)

The MRPS16 Polyclonal Antibody (CAB9874) is a valuable tool for researchers studying mitochondrial ribosomal protein S16 (MRPS16), a key component of the mitochondrial ribosome involved in protein synthesis within the mitochondria. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in various research applications, including Western blotting.MRPS16 is essential for proper mitochondrial function and has been implicated in various diseases, including mitochondrial disorders and cancer.

By targeting MRPS16 with this polyclonal antibody, researchers can investigate its role in mitochondrial protein synthesis and potential implications for disease development.Understanding the function of MRPS16 is crucial for unraveling the intricacies of mitochondrial biology and its impact on human health. With the MRPS16 Polyclonal Antibody, researchers can delve deeper into the mechanisms underlying mitochondrial protein synthesis and its implications for disease pathology.

Antibody Name:Anti-MRPS16 Antibody
Antibody SKU:CAB9874
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human MRPS16 (NP_057149.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, SW620, HT-1080, MCF7, Mouse heart, Mouse kidney, Mouse liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human MRPS16 (NP_057149.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVHL TTLL CKAY RGGH LTIR LALG GCTN RPFY RIVA AHNK CPRD GRFV EQLG SYDP LPNS HGEK LVAL NLDR IRHW IGCG AHLS KPME KLLG LAGF FPLH PMMI TNAE RLRR KRAR EVLL ASQK TDAE ATDT EATE T
Gene ID:51021
Uniprot:Q9Y3D3
Cellular Location:Mitochondrion
Calculated MW:13kDa/15kDa
Observed MW:15kDa
Synonyms:MRPS16, CGI-132, COXPD2, MRP-S16, RPMS16
Background:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described.
UniProt Protein Function:MRPS16: Defects in MRPS16 are the cause of combined oxidative phosphorylation deficiency type 2 (COXPD2). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD2 symptoms include fatal neonatal metabolic acidosis with agenesis of the corpus callosum. Belongs to the ribosomal protein S16P family.
UniProt Protein Details:

Protein type:Mitochondrial; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; mitochondrial small ribosomal subunit; cytoplasm; mitochondrial inner membrane

Molecular Function:protein binding; structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 2

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y3D3
NCBI GenInfo Identifier:7705626
NCBI Gene ID:51021
NCBI Accession:NP_057149.1
UniProt Secondary Accession:Q9Y3D3,Q96Q60, B4E032,
UniProt Related Accession:Q9Y3D3
Molecular Weight:13,829 Da
NCBI Full Name:28S ribosomal protein S16, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein S16
NCBI Official Symbol:MRPS16  
NCBI Official Synonym Symbols:COXPD2; RPMS16; CGI-132; MRP-S16  
NCBI Protein Information:28S ribosomal protein S16, mitochondrial
UniProt Protein Name:28S ribosomal protein S16, mitochondrial
Protein Family:28S ribosomal protein
UniProt Gene Name:MRPS16  
UniProt Entry Name:RT16_HUMAN
Anti-MRPS16 Polyclonal Antibody (CAB9874)Western blot analysis of extracts of various cell lines, using MRPS16 antibody (CAB9874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-MRPS16 Polyclonal Antibody (CAB9874)Confocal immunofluorescence analysis of U2OS cells using Anti-MRPS16 Polyclonal Antibody (CAB9874) at dilution of 1:100. Blue: DAPI for nuclear staining.

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