The MRPS14 Polyclonal Antibody (CAB15498) is a valuable tool for researchers studying MRPS14, a mitochondrial ribosomal protein known for its role in protein synthesis within the mitochondria. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the MRPS14 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and mitochondrial research.MRPS14 is an essential component of the mitochondrial ribosome and plays a crucial role in the translation of mitochondrial DNA-encoded proteins, ultimately contributing to the maintenance of mitochondrial function and cellular metabolism.
Research into MRPS14 is particularly relevant in the context of mitochondrial dysfunction, metabolic disorders, and aging-related diseases where mitochondrial protein synthesis may be impaired. By elucidating the function and regulation of MRPS14, researchers can gain insights into the molecular mechanisms underlying these pathological conditions, potentially leading to the development of novel therapeutic strategies.
Product Name:
MRPS14 Rabbit Polyclonal Antibody
SKU:
CAB15498
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
63931
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS14 Rabbit pAb (CAB15498) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
