The MRPS12 Polyclonal Antibody (CAB10573) is a valuable tool for researchers studying the mitochondrial ribosomal protein S12 (MRPS12) and its role in cellular processes. This antibody, produced in rabbits, is highly specific for human samples and is suitable for use in Western blot applications.MRPS12 is a crucial component of the mitochondrial ribosome, which is responsible for protein synthesis within the mitochondria. Dysregulation of MRPS12 has been linked to a variety of disorders, including neurodegenerative diseases and metabolic disorders. By using the MRPS12 Polyclonal Antibody, researchers can detect and analyze MRPS12 expression levels in various cell types, allowing for further investigation into its functions and potential therapeutic targets.
With its high reactivity and specificity, the MRPS12 Polyclonal Antibody is an essential tool for scientists involved in molecular biology, biochemistry, and mitochondrial research. Its ability to target MRPS12 with precision makes it a valuable asset in advancing our understanding of mitochondrial function and its implications in human health and disease.
Product Name:
MRPS12 Rabbit Polyclonal Antibody
SKU:
CAB10573
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-138 of human MRPS12 (NP_203527.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.
Purification Method:
Affinity purification
Gene ID:
6183
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS12 Rabbit pAb (CAB10573) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
