The MRPL45 Polyclonal Antibody (CAB13197) is a valuable tool for researchers studying MRPL45, a mitochondrial ribosomal protein essential for protein synthesis in mitochondria. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for a variety of applications including Western blot analysis.MRPL45 is known to play a crucial role in maintaining mitochondrial function and overall cell health. Dysregulation of MRPL45 has been linked to various diseases such as cancer and mitochondrial disorders, highlighting the importance of studying this protein in different biological contexts.
By targeting MRPL45, researchers can gain insights into mitochondrial protein synthesis and its impact on cellular processes. This antibody enables the detection and quantification of MRPL45 in different cell types, providing valuable information for studies in molecular biology, cell biology, and mitochondrial dysfunction research. Unlock the potential of your research with the MRPL45 Polyclonal Antibody.
Product Name:
MRPL45 Rabbit Polyclonal Antibody
SKU:
CAB13197
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.
Purification Method:
Affinity purification
Gene ID:
84311
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL45 Rabbit pAb (CAB13197) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
