The MRPL43 Polyclonal Antibody (CAB17793) is a valuable tool for research involving MRPL43, a protein located in the mitochondria that plays a crucial role in protein synthesis. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot and immunohistochemistry applications. It binds specifically to the MRPL43 protein, allowing for accurate detection and analysis in various cell types.MRPL43 is a key component of the mitochondrial ribosome and is essential for the translation of protein in the mitochondria. Its role in protein synthesis makes it a crucial target for research in areas such as mitochondrial biology, metabolism, and aging.
Understanding the function of MRPL43 can provide insights into mitochondrial dysfunction and its implications in various diseases, including neurodegenerative disorders and metabolic syndromes.Overall, the MRPL43 Polyclonal Antibody (CAB17793) is a valuable tool for researchers studying mitochondrial biology and protein synthesis. Its high specificity and reactivity make it a reliable choice for investigating the role of MRPL43 in various cellular processes and disease pathways.
Product Name:
MRPL43 Rabbit Polyclonal Antibody
SKU:
CAB17793
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-155 of human MRPL43 (NP_789762.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms.
Purification Method:
Affinity purification
Gene ID:
84545
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL43 Rabbit pAb (CAB17793) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
