The MRPL32 Polyclonal Antibody (CAB10016) is a valuable tool for researchers studying MRPL32, a mitochondrial ribosomal protein involved in protein synthesis within the mitochondria. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is specifically validated for Western blot applications. By binding to the MRPL32 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and mitochondrial research.MRPL32 is essential for the maintenance of mitochondrial function and plays a crucial role in the synthesis of proteins necessary for proper mitochondrial activity.
Research on MRPL32 is important for understanding mitochondrial protein synthesis and its impact on cellular processes such as energy production and cell growth. This antibody provides a reliable tool for investigating the function of MRPL32 in health and disease, offering insights into mitochondrial dysfunction and potential therapeutic targets for mitochondrial-related disorders.
Product Name:
MRPL32 Rabbit Polyclonal Antibody
SKU:
CAB10016
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
Purification Method:
Affinity purification
Gene ID:
64983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MRPL32 antibody (CAB10016) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
