The MRPL30 Polyclonal Antibody (CAB16529) is a valuable tool for researchers studying MRPL30, a mitochondrial ribosomal protein critical for protein synthesis within the mitochondria. This antibody is produced in rabbits and is specifically designed to detect MRPL30 in human samples, making it ideal for various research applications, including Western blotting.MRPL30 is essential for the proper functioning of the mitochondria and is involved in the synthesis of proteins necessary for mitochondrial function. Research into MRPL30 can provide insights into mitochondrial protein synthesis, cellular metabolism, and mitochondrial dysfunction, which are important in understanding various diseases, including metabolic disorders and cancer.
By using the MRPL30 Polyclonal Antibody, researchers can accurately detect and analyze MRPL30 levels in different cell types, allowing for further exploration into the role of this protein in mitochondrial function and disease pathology. This antibody is a valuable tool for studies in molecular biology, cell biology, and mitochondrial research, offering a deeper understanding of MRPL30 and its implications in health and disease.
Product Name:
MRPL30 Rabbit Polyclonal Antibody
SKU:
CAB16529
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.
Purification Method:
Affinity purification
Gene ID:
51263
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL30 Rabbit pAb (CAB16529) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
