The MRPL28 Polyclonal Antibody (CAB0452) from AssayGenie is a valuable tool for researchers studying MRPL28, a mitochondrial ribosomal protein known for its role in protein synthesis within the mitochondria. This antibody, raised in rabbits, has high reactivity with human samples and has been validated for use in various applications, including Western blotting.MRPL28 is essential for maintaining mitochondrial function and mutations in this gene have been linked to mitochondrial disorders and diseases.
By targeting MRPL28 with this antibody, researchers can analyze its expression levels and localization in different cell types, providing insights into its function and potential implications in mitochondrial-related diseases.Through the use of the MRPL28 Polyclonal Antibody (CAB0452), researchers can enhance their studies in mitochondrial biology, biochemistry, and related fields, ultimately contributing to a better understanding of mitochondrial protein synthesis and its role in health and disease.
Product Name:
MRPL28 Rabbit Monoclonal Antibody
SKU:
CAB0452
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MRPL28 (NP_006419.2).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Purification Method:
Affinity purification
Gene ID:
10573
Clone Number:
ARC2507
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL28 Rabbit mAb (CAB0452) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
